| Catalog No. | Size | Price (USD) |
| 202274-T36-50 | 50 µL | $147.96 |
| 202274-T36-100 | 100 µL | $251.64 |
| 202274-T36-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Bcl-2 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Bcl-2 |
| Immunogen | E. coli-derived Human Bcl-2 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human Bcl-2 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-Bcl-2 Antibody; Anti-PPP1R50 Antibody
Image / Experimental Information
| Image 1 Description | Anti-Bcl2 rabbit polyclonal antibody at 1:500 dilution Lane A: Raji Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:26 kDa |
| Image 1 Alt Text | Human Bcl-2 Western blot (WB) 22368 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-Bcl2 rabbit polyclonal antibody at 1:500 dilution Lane A: Raji Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:26 kDa Observed band size:26 kDa |
| Image 2 Alt Text | Human Bcl-2 Immunoprecipitation(IP) 22369 |
| Image 2 URL | Source image |
Background Information
| Full Name | B-cell CLL/lymphoma 2 |
| Description | BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. |
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