| Catalog No. | Size | Price (USD) |
| 14321-T52-50 | 50 µL | $147.96 |
| 14321-T52-100 | 100 µL | $251.64 |
| 14321-T52-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-ENSA Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human ENSA |
| Immunogen | Recombinant Human ENSA / Endosulfine alpha protein (Catalog#14321-H07E) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human ENSA / Endosulfine alpha (rh ENSA / Endosulfine alpha; Catalog#14321-H07E; O43768-1; Met1-Glu121). ENSA / Endosulfine alpha specific IgG was purified by Human ENSA / Endosulfine alpha affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ARPP-19e Antibody; Anti-MGC4319 Antibody; Anti-MGC78563 Antibody; Anti-MGC8394 Antibody
Image / Experimental Information
| Image 1 Description | Anti-ENSA rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse heart tissue lysate Whole Cell Lysate Lane B: Rat heart tissue lysate Whole Cell Lysate Lane C: Mouse brain tissue lysate Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:26 kDa |
| Image 1 Alt Text | Human ENSA / Endosulfine alpha Western blot (WB) 13575 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-ENSA rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse heart tissue lysate Whole Cell Lysate Lane B: Rat heart tissue lysate Whole Cell Lysate Lane C: Mouse brain tissue lysate Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:26 kDa |
| Image 2 Alt Text | Human ENSA / Endosulfine alpha Immunoprecipitation(IP) 15287 |
| Image 2 URL | Source image |
Background Information
| Full Name | endosulfine alpha |
| Description | Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents. |
| References |
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