| Catalog No. | Size | Price (USD) |
| 201473-T38-50 | 50 µL | $147.96 |
| 201473-T38-100 | 100 µL | $251.64 |
| 201473-T38-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Beclin 1/BECN1 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Beclin 1/BECN1 |
| Immunogen | E. coli-derived Human Beclin 1/BECN1 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human Beclin 1/BECN1 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ATG6 Antibody; Anti-beclin1 Antibody; Anti-GT197 Antibody; Anti-VPS30 Antibody
Image / Experimental Information
| Image 1 Description | Anti-BECN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:60 kDa Observed band size:60 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Human Beclin 1/BECN1 Western blot (WB) 29462 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-BECN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:60 kDa Observed band size:60 kDa (We are unsure as to the identity of these extra bands.) |
| Image 2 Alt Text | Human Beclin 1/BECN1 Immunofluorescence(IF) 29463 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-BECN1 rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:60 kDa Observed band size:60 kDa (We are unsure as to the identity of these extra bands.) |
| Image 3 Alt Text | Human Beclin 1/BECN1 Immunoprecipitation(IP) 29464 |
| Image 3 URL | Source image |
Background Information
| Full Name | beclin 1, autophagy related |
| Description | BECN1 (Beclin 1), a highly conserved eukaryotic protein, is a key regulator of autophagy, a cellular homeostasis pathway, and also participates in vacuolar protein sorting, endocytic trafficking, and apoptosis. BECN1 is important for embryonic development, the innate immune response, tumor suppression, and protection against neurodegenerative disorders, diabetes, and heart disease. BECN1 mediates autophagy as a core component of the class III phosphatidylinositol 3-kinase complexes.The decreased mRNA expression of the autophagy gene BECN1 may contribute to the pathogenesis and progression of HER2-enriched, basal-like, and TP53 mutant breast cancers. BECN1/Beclin 1 is regarded as a critical component in the class III phosphatidylinositol 3-kinase (PtdIns3K) complex to trigger autophagy in mammalian cells. Despite its significant role in a number of cellular and physiological processes, the exact function of BECN1 in autophagy remains controversial. |
| Research Areas |
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| Related Pathways |
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title: Copper toxicity in the liver of broiler chicken: insights from metabolomics and AMPK-mTOR mediated autophagy perspective.
authors: Jing Chen; Jianzhao Liao; ...; Fan Yang
journal: Poultry science
date: 2024-06-22
pmid: 38991386
doi: 10.1016/j.psj.2024.104011
cited_by_count: 11
techniques: Control; Cytometry; Immunohistochemical staining; Incubation; Software; Staining; Western Blot
bioz_score: 92
title: Copper induces energy metabolic dysfunction and AMPK-mTOR pathway-mediated autophagy in kidney of broiler chickens.
authors: Jianzhao Liao; Fan Yang; ...; Zhaoxin Tang
journal: Ecotoxicology and Environmental Safety
date: 2020-12-18
pmid: 33010598
doi: 10.1016/j.ecoenv.2020.111366
cited_by_count: 51
techniques: Control; Expressing; Immunohistochemical staining; Immunohistochemistry; Incubation; Microscopy; Staining; Western Blot
bioz_score: 92
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