| Catalog No. | Size | Price (USD) |
| 11582-RP01-20 | 20 µL | $88.87 |
| 11582-RP01-100 | 100 µL | $192.40 |
| 11582-RP01-200 | 200 µL | $251.64 |
| 11582-RP01-400 | 400 µL | $355.32 |
General Information
| Product name | Anti-BirA Antibody, Rabbit Polyclonal |
| Validated applications | ELISA (FAQ Protocol) |
| Application notes | (Antibody's applications have not been validated with corresponding E. coli. Optimal concentrations/dilutions should be determined by the end user.) |
| Species reactivity | Reacts with: E. coli |
| Specificity | E. coli BirA |
| Immunogen | Recombinant E. coli BirA / Bifunctional protein BirA protein (Catalog#11582-E10E) |
| Preparation | Produced in rabbits immunized with purified, recombinant E. coli BirA / Bifunctional protein BirA (Catalog#11582-E10E; P06709; Met1-Lys321). Total IgG was purified by Protein A affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | The enzyme BirA is a key reagent because of its ability to biotinylate proteins at a specific residue in a recognition sequence. This enzyme is used to biotinylate the C termini of membrane proteins, allowing these proteins to be tetramerized by binding to streptavidin. Because of the specificity of the biotinylation at the C terminus, the orientation of the membrane proteins on the streptavidin is equivalent to that of the native protein on the cell surface. These tetrameric proteins can be used to study protein receptor-ligand interactions at the cell surface, and site-specific biotinylation can be used to study proteins in vitro using a defined orientation. The biotinylation of histones by BirA ligase is consistent with the proposed role of human HCS in chromatin. The N-terminal BirA domain is required for both transcriptional regulation of biotin synthesis and biotin protein ligase activity. |
title: Proximity labelling of pro-interleukin-1α reveals evolutionary conserved nuclear interactions
authors: Rose Wellens; Victor S. Tapia; ...; Christopher Hoyle
journal: Nature Communications
date: 2024-08-08
pmid: 39117622
pmcid: 11310415
cited_by_count: 4
techniques: Construct; Fluorescence; Generated; Immunofluorescence; Microscopy; Staining; Transfection; Western Blot
bioz_score: 93
title: Sperm-Associated Antigen 9 Promotes Influenza A Virus-Induced Cell Death via the c-Jun N-Terminal Kinase Signaling Pathway
authors: Rui Gui; Huabin Zheng; ...; Yung-Fu Chang
journal: mBio
date: 2022-05-31
pmid: 35638835
doi: 10.1128/mbio.00615-22
pmcid: 9239253
cited_by_count: 6
techniques: Construct; Labeling; Plasmid Preparation; Transfection
bioz_score: 93
title: A Toolbox for Efficient Proximity-Dependent Biotinylation in Zebrafish Embryos
authors: Shimon M. Rosenthal; Tvisha Misra; ...; Anne-Claude Gingras
journal: Molecular & cellular proteomics : MCP
date: 2021-07-29
pmid: 34332124
doi: 10.1016/j.mcpro.2021.100128
pmcid: 8383115
cited_by_count: 21
techniques: BIA-KA; Blocking Assay; Protein Concentration; SDS Page; Software; Western Blot
bioz_score: 93
