| Catalog No. | Size | Price (USD) |
| 10445-RP02-50 | 50 µL | $147.96 |
| 10445-RP02-100 | 100 µL | $251.64 |
| 10445-RP02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-FLT3 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human FLT3 |
| Immunogen | Recombinant Human FLT3 protein (Catalog#10390-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human FLT3 (rh FLT3; Catalog#10390-H08H; AAL65157.1; Met 1-Arg 200). FLT3 specific IgG was purified by human FLT3 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CD135 Antibody; Anti-FLK-2 Antibody; Anti-FLK2 Antibody; Anti-STK1 Antibody
Image / Experimental Information
| Image 1 Description | FLT3 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg k562 Whole Cell Lysate 2 µL anti-FLT3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-FLT3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDa |
| Image 1 Alt Text | Human FLT3/CD135/FLK-2 Immunoprecipitation(IP) 7986 |
| Image 1 URL | Source image |
| Image 2 Description | FLT3 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg k562 Whole Cell Lysate 2 µL anti-FLT3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-FLT3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDa |
| Image 2 Alt Text | Human FLT3/CD135/FLK-2 Western blot (WB) 13219 |
| Image 2 URL | Source image |
Background Information
| Full Name | fms-related tyrosine kinase 3 |
| Description | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeⅢ for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia. |
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