| Catalog No. | Size | Price (USD) |
| 100242-MM05-20 | 20 µL | $118.49 |
| 100242-MM05-50 | 50 µL | $148.11 |
| 100242-MM05-100 | 100 µL | $236.98 |
General Information
| Product name | GAPDH Loading Control Antibody, Mouse Mab |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Mouse, Rat (Species predicted to react based on 100% sequence homology) |
| Immunogen | A synthetic peptide corresponding to the center region of the human GAPDH |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human GAPDH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #05 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CDABP0047 Antibody; Anti-G3PD Antibody; Anti-GAPD Antibody; Anti-HEL-S-162eP Antibody; Anti-OK/SW-cl.12 Antibody
Image / Experimental Information
| Image 1 Description | Anti-GAPDH mouse monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: MCF7 whole cell lysate Lane E: PC-3 whole cell lysate Lane F: Daudi whole cell lysate Lane G: A549 whole cell lysate Lane H: Rat heart tissue lysate Lane I: Rat liver tissue lysate Lane J: Rat spleen tissue lysate Lane K: Rat lung tissue lysate Lane L: Rat kidney tissue lysate Lane M: Rat brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: kDa Observed band size:38 kDa |
| Image 1 Alt Text | Human GAPDH Western blot (WB) 6437 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-GAPDH mouse monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: MCF7 whole cell lysate Lane E: PC-3 whole cell lysate Lane F: Daudi whole cell lysate Lane G: A549 whole cell lysate Lane H: Rat heart tissue lysate Lane I: Rat liver tissue lysate Lane J: Rat spleen tissue lysate Lane K: Rat lung tissue lysate Lane L: Rat kidney tissue lysate Lane M: Rat brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: kDa Observed band size:38 kDa |
| Image 2 Alt Text | Human GAPDH Immunohistochemistry(IHC) 24113 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-GAPDH mouse monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lane D: MCF7 whole cell lysate Lane E: PC-3 whole cell lysate Lane F: Daudi whole cell lysate Lane G: A549 whole cell lysate Lane H: Rat heart tissue lysate Lane I: Rat liver tissue lysate Lane J: Rat spleen tissue lysate Lane K: Rat lung tissue lysate Lane L: Rat kidney tissue lysate Lane M: Rat brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: kDa Observed band size:38 kDa |
| Image 3 Alt Text | Human GAPDH Immunohistochemistry(IHC) 24114 |
| Image 3 URL | Source image |
Background Information
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Description | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. |
| Research Areas |
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| References |
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title: Modular Nanoassemblies Mimicking p62 Aggregates for Targeted Organelle Sequestration and Degradation against Breast Cancer.
authors: Yuai Li; Yunting Zhang; ...; Yao Fu
journal: ACS Nano
date: 2025-10-08
pmid: 41063366
doi: 10.1021/acsnano.5c10801
cited_by_count: 0
techniques: Immunofluorescence; Immunohistochemistry; Western Blot
bioz_score: 93
title: CHMP4A in hepatocellular carcinoma: exploring its role in tumor progression, immune modulation, and potential link to TIM3 checkpoint
authors: Kai Sun; Song Wen; ...; Ke-run Wang
journal: Frontiers in Immunology
date: 2025-10-02
doi: 10.3389/fimmu.2025.1682724
cited_by_count: 0
techniques: Binding Assay; Biomarker Discovery; CCK-8 Assay; Control; Electrophoresis; Expressing; Knockdown; Migration; Negative Control; Quantitative RT-PCR; Transwell Assay; Viability Assay; Virus; Western Blot
bioz_score: 93
title: Leveraging T cell-specific fusogenicity of HIV for in vivo mRNA delivery to produce human CAR-T cells
authors: Yue Wang; Min Yang; ...; Jun Wang
journal: Cell Biomaterials
date: 2025-03-01
doi: 10.1016/j.celbio.2025.100011
cited_by_count: 5
techniques: CCK-8 Assay; Enzyme-linked Immunosorbent Assay; Flow Cytometry; Western Blot
bioz_score: 93
title: The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway.
authors: Linping Lei; Xuqin Xie; ...; Zongguang Zhou
journal: Investigational new drugs
date: 2022-02-02
pmid: 32981006
doi: 10.1007/s10637-020-01014-0
cited_by_count: 8
techniques: Apoptosis Assay; Centrifugation; Electrophoresis; Incubation; Lysis; Protease Inhibitor; SDS Page; Software; Western Blot
bioz_score: 93
title: Intranuclear cardiac troponin I plays a functional role in regulating Atp2a2 expression in cardiomyocytes
authors: Qian Lu; Bo Pan; ...; Jie Tian
journal: Genes & Diseases
date: 2021-05-15
pmcid: 9485201
cited_by_count: 5
techniques: Protein Concentration; Protein Extraction; Protein Quantitation; SDS Page; Western Blot
bioz_score: 93
title: Complement 1q protects MRL/lpr mice against lupus nephritis via inhibiting the nuclear factor-κB pathway.
authors: Juanjuan Sun; Shuxia Guo; ...; Yan Zhuang
journal: Molecular medicine reports
date: 2021-05-10
pmid: 33173997
doi: 10.3892/mmr.2020.11588
cited_by_count: 6
techniques: Control
bioz_score: 93
title: ERK5‑regulated RERG expression promotes cancer progression in prostatic carcinoma.
authors: Ying Xiong; Hui Huang; ...; Luying Zhang
journal: Oncology Reports
date: 2020-09-11
pmid: 30431143
doi: 10.3892/or.2018.6852
cited_by_count: 7
bioz_score: 93
title: Selection of viral variants during persistent infection of insectivorous bat cells with Middle East respiratory syndrome coronavirus
authors: Arinjay Banerjee; Sonu Subudhi; ...; Vikram Misra
journal: Scientific Reports
date: 2020-04-29
pmid: 32350357
doi: 10.1038/s41598-020-64264-1
pmcid: 7190632
cited_by_count: 19
techniques: Agarose Gel Electrophoresis; Blocking Assay; Concentration Assay; Construct; Expressing; Fluorescence; Immunofluorescence; Incubation; Infection; Marker; Microscopy; Mutagenesis; Negative Control; Quantitative RT-PCR; Real-time Polymerase Chain Reaction; Sequencing; Staining; Transfection; Western Blot
bioz_score: 93
title: Difluoromethylornithine, a Decarboxylase 1 Inhibitor, Suppresses Hepatitis B Virus Replication by Reducing HBc Protein Levels
authors: Binli Mao; Zhuo Wang; ...; Yuan Hu
journal: Frontiers in cellular and infection microbiology
date: 2020-04-16
pmid: 32373551
doi: 10.3389/fcimb.2020.00158
pmcid: 7176913
cited_by_count: 9
techniques: Software; Western Blot
bioz_score: 93
title: Potential Markers from Serum-Purified Exosomes for Detecting Oral Squamous Cell Carcinoma Metastasis
authors: Cuiping Li; Yang Zhou; ...; Nuo Zhou
journal: Cancer Epidemiology Biomarkers & Prevention
date: 2019-07-26
doi: 10.1158/1055-9965.epi-18-1122
cited_by_count: 39
techniques: Enzyme-linked Immunosorbent Assay; Expressing; Immunohistochemistry; Western Blot
bioz_score: 93
title: The Anti-proliferative Activity of GnRH Through Downregulation of the Akt/ERK Pathways in Pancreatic Cancer
authors: Linna Suo; Xiaocen Chang; ...; Hongmei Ji
journal: Frontiers in Endocrinology
date: 2019-06-17
pmid: 31263453
doi: 10.3389/fendo.2019.00370
pmcid: 6590102
cited_by_count: 12
techniques: Fluorescence; SDS Page; Software; Western Blot
bioz_score: 93
title: The Orexin-A-Regulated Akt/mTOR Pathway Promotes Cell Proliferation Through Inhibiting Apoptosis in Pancreatic Cancer Cells
authors: Linna Suo; Xiaocen Chang; Yuyan Zhao
journal: Frontiers in Endocrinology
date: 2018-10-31
pmid: 30429828
doi: 10.3389/fendo.2018.00647
pmcid: 6220114
cited_by_count: 19
bioz_score: 93
title: Escherichia coli responds to environmental changes using enolasic degradosomes and stabilized DicF sRNA to alter cellular morphology
authors: Oleg N. Murashko; Sue Lin-Chao
journal: Proceedings of the National Academy of Sciences of the United States of America
date: 2017-09-05
pmid: 28874523
doi: 10.1073/pnas.1703731114
pmcid: 5617263
cited_by_count: 80
techniques: Autoradiography; Blocking Assay; Control; Imaging; Incubation; Membrane; Saline; Western Blot
bioz_score: 93
title: Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse
authors: Chongyang Wu; Ying Zhang; ...; Jinlian Hua
journal: Oncotarget
date: 2016-12-13
pmid: 27806317
doi: 10.18632/oncotarget.12990
pmcid: 5347676
cited_by_count: 16
techniques: Imaging; Lysis; Protein Concentration; Western Blot
bioz_score: 93
title: Vinculin and Rab5 Complex Is Requited for Uptake of Staphyrococcus aureus and Interleukin-6 Expression
authors: Makoto Hagiwara; Eitoyo Kokubu; ...; David M. Ojcius
journal: PLoS ONE
date: 2014-01-23
pmid: 24466349
doi: 10.1371/journal.pone.0087373
pmcid: 3900708
cited_by_count: 21
techniques: Control; Incubation; Infection; Knockdown; Labeling; Transfection; Western Blot
bioz_score: 93
