| Catalog No. | Size | Price (USD) |
| 10345-T48-50 | 50 µL | $147.96 |
| 10345-T48-100 | 100 µL | $251.64 |
| 10345-T48-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Granzyme B Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Granzyme B |
| Immunogen | Recombinant Human Granzyme B/GZMB Protein (Catalog#10345-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human Granzyme B/GZMB (rh Granzyme B/GZMB; Catalog#10345-H08H; NP_004122.1; Met1-Tyr247). Granzyme B/GZMB specific IgG was purified by Human Granzyme B/GZMB affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-C11 Antibody; Anti-CCPI Antibody; Anti-CGL-1 Antibody; Anti-CGL1 Antibody; Anti-CSP-B Antibody; Anti-CSPB Antibody; Anti-CTLA1 Antibody; Anti-CTSGL1 Antibody; Anti-GRB Antibody; Anti-HLP Antibody; Anti-SECT Antibody
Image / Experimental Information
| Image 1 Description | Anti-GranzymeB rabbit polyclonal antibody at 1:500 dilution Lane A: HL-60 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Human Granzyme B Western blot (WB) 20231 |
| Image 1 URL | Source image |
Background Information
| Full Name | granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
| Description | Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD, and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance. |
| Research Areas |
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| References |
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title: The binding of an anti-PD-1 antibody to FcγRΙ has a profound impact on its biological functions
authors: Tong Zhang; Xiaomin Song; ...; Kang Li
journal: Cancer Immunology, Immunotherapy
date: 2018-04-23
pmid: 29687231
doi: 10.1007/s00262-018-2160-x
pmcid: 6006217
cited_by_count: 192
techniques: Immunofluorescence; Membrane; Staining
bioz_score: 90
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