| Catalog No. | Size | Price (USD) |
| 100005-MM01-20 | 20 µL | $88.87 |
| 100005-MM01-50 | 50 µL | $118.49 |
| 100005-MM01-100 | 100 µL | $177.73 |
General Information
| Product name | Anti-Histone H3 Antibody, Mouse Monoclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Mouse, Rat (Species predicted to react based on 100% sequence homology) |
| Immunogen | A synthetic peptide corresponding to the C-terminus of the human Histone H3. |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG2b Clone #1F6D7C4 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. |
| Image 1 Alt Text | Human Histone H3 Immunohistochemistry(IHC) 4056 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. |
| Image 2 Alt Text | Human Histone H3 Immunohistochemistry(IHC) 4057 |
| Image 2 URL | Source image |
| Image 3 Description | Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. |
| Image 3 Alt Text | Human Histone H3 Western blot (WB) 13023 |
| Image 3 URL | Source image |
| Image 4 Description | Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. |
| Image 4 Alt Text | Human Histone H3 Western blot (WB) 20381 |
| Image 4 URL | Source image |
| Image 5 Description | Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. |
| Image 5 Alt Text | Human Histone H3 Western blot (WB) 20382 |
| Image 5 URL | Source image |
Background Information
| Description | Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes. |
| References |
|
title: Comparative Quantitative Profiling of Protein Lactylation Reveals a Dynamic Tissues-Specific Network Associated with Metabolic Specialization in Yaks
authors: Zhijuan Wu; Huan Yang; ...; Zhixin Chai
journal: Animals : An Open Access Journal From Mdpi
date: 2026-07-18
pmid: 42511105
doi: 10.3390/ani16142228
cited_by_count: 0
techniques: Incubation; Western Blot
bioz_score: 94
title: Construction of a clickable probe‐based protein chip platform for discovering covalent mIDH1 inhibitors from natural medicinal extracts
journal: iMeta
date: 2026-02-12
doi: 10.1002/imt2.70107
cited_by_count: 5
techniques: Bicinchoninic Acid Protein Assay; Imaging; Incubation; Lysis; Protease Inhibitor; Protein Concentration; Software; Western Blot
bioz_score: 94
title: Nuclear translocation of Sp100A suppresses multiple DNA viruses and limits HSV-1 lytic replication in vivo.
authors: Yilei Ma; Weidong Li; ...; Pei Xu
journal: Virologica Sinica
date: 2026-02-05
pmid: 41654192
doi: 10.1016/j.virs.2026.02.003
cited_by_count: 0
techniques: Clone Assay; Protease Inhibitor; Sequencing; Transfection
bioz_score: 94
title: ACSS2-mediated acetyl-CoA accumulation promotes mitophagy and tumor growth via increased H3K27ac in HBV-related HCC.
authors: Shan Li; Jie Hu; ...; Ni Tang
journal: Clinical and molecular hepatology
date: 2025-12-19
pmid: 41414763
doi: 10.3350/cmh.2025.0754
cited_by_count: 0
bioz_score: 94
title: Broad mucosal and systemic immunity in mice induced by intranasal booster with a novel recombinant adenoviral based vaccine protects against divergent influenza A virus
journal: Authorea
date: 2025-01-05
doi: 10.22541/au.173610176.62554351/v1
cited_by_count: 0
techniques: Incubation; Membrane; Western Blot
bioz_score: 94
title: Capture of RNA-binding proteins across mouse tissues using HARD-AP
authors: Yijia Ren; Hongyu Liao; ...; Zhihong Xue
journal: Nature Communications
date: 2024-09-28
pmid: 39341811
pmcid: 11438895
cited_by_count: 1
techniques: Clone Assay
bioz_score: 94
title: Inhibiting HMGCR represses stemness and metastasis of hepatocellular carcinoma via Hedgehog signaling
authors: Zhirong Zhang; Jiayao Yang; ...; Ni Tang
journal: Genes & Diseases
date: 2024-04-03
doi: 10.1016/j.gendis.2024.101285
pmcid: 11252768
cited_by_count: 0
techniques: Incubation; Membrane; Western Blot
bioz_score: 94
title: Enhanced glycolysis-mediated energy production in alveolar stem cells is required for alveolar regeneration.
authors: Zheng Wang; Dongdong Wei; ...; Nan Tang
journal: Cell Stem Cell
date: 2023-08-07
pmid: 37541209
doi: 10.1016/j.stem.2023.07.007
cited_by_count: 40
techniques: Protease Inhibitor; Western Blot
bioz_score: 94
title: Gluconeogenic enzyme PCK1 supports S-adenosylmethionine biosynthesis and promotes H3K9me3 modification to suppress hepatocellular carcinoma progression
authors: Dongmei Gou; Rui Liu; ...; Ni Tang
journal: The Journal of Clinical Investigation
date: 2023-07-03
pmid: 37166978
doi: 10.1172/JCI161713
pmcid: 10313362
cited_by_count: 26
bioz_score: 94
title: HSV-1 selectively packs the transcription factor Oct-1 into EVs to facilitate its infection.
authors: Yilei Ma; Xiaomei Deng; ...; Pei Xu
journal: Frontiers in Microbiology
date: 2023-04-14
pmid: 37396389
cited_by_count: 5
techniques: CRISPR; Control; Protease Inhibitor; Transfection
bioz_score: 93
title: Characterization of a KDM5 small molecule inhibitor with antiviral activity against hepatitis B virus
authors: Sarah A. Gilmore; Danny Tam; ...; Haitao Guo
journal: PLoS ONE
date: 2022-12-07
pmcid: 9728921
cited_by_count: 0
techniques: Enzyme-linked Immunosorbent Assay; Incubation
bioz_score: 94
title: Super-enhancers conserved within placental mammals maintain stem cell pluripotency
authors: Juqing Zhang; Yaqi Zhou; ...; Jinlian Hua
journal: Proceedings of the National Academy of Sciences of the United States of America
date: 2022-09-26
pmid: 36161929
doi: 10.1073/pnas.2204716119
pmcid: 9546576
cited_by_count: 19
bioz_score: 94
title: Crosstalk of Brucella abortus nucleomodulin BspG and host DNA replication process/mitochondrial respiratory pathway promote anti-apoptosis and infection.
authors: Zhongchen Ma; Xiaoyu Deng; ...; Chuangfu Chen
journal: Veterinary Microbiology
date: 2022-04-27
pmid: 35395545
doi: 10.1016/j.vetmic.2022.109414
cited_by_count: 8
techniques: Expressing; Radio Immunoprecipitation; Transfection
bioz_score: 94
title: Mutation of Signal Transducer and Activator of Transcription 5 (STAT5) Binding Sites Decreases Milk Allergen α S1 -Casein Content in Goat Mammary Epithelial Cells
authors: Ning Song; Jun Luo; ...; Michelle Colgrave
journal: Foods
date: 2022-01-26
pmid: 35159497
doi: 10.3390/foods11030346
pmcid: 8834060
cited_by_count: 8
techniques: Activity Assay; Binding Assay; Expressing; Incubation; Lysis; Protease Inhibitor; Protein Concentration; SDS Page; Transfection; Western Blot
bioz_score: 94
title: VPS34 suppression reverses osimertinib resistance via simultaneously inhibiting glycolysis and autophagy.
authors: Hengyi Chen; Conghua Lu; ...; Yong He
journal: Carcinogenesis
date: 2021-11-22
pmid: 33848354
doi: 10.1093/carcin/bgab030
cited_by_count: 8
techniques: Cell Culture; Incubation; MTT Assay
bioz_score: 94
title: Point mutation impairs centromeric CENH3 loading and induces haploid plants
authors: Raheleh Karimi-Ashtiyani; Takayoshi Ishii; ...; Andreas Houben
journal: Proceedings of the National Academy of Sciences of the United States of America
date: 2015-09-08
pmid: 26294252
doi: 10.1073/pnas.1504333112
pmcid: 4568683
cited_by_count: 124
techniques: Incubation
bioz_score: 94
title: Design, synthesis, and structure-activity relationship of novel LSD1 inhibitors based on pyrimidine-thiourea hybrids as potent, orally active antitumor agents.
authors: Li-Ying Ma; Yi-Chao Zheng; ...; Hong-Min Liu
journal: Journal of Medicinal Chemistry
date: 2015-05-07
pmid: 25610955
doi: 10.1021/acs.jmedchem.5b00037
cited_by_count: 138
techniques: Purification; Western Blot
bioz_score: 94
title: Triazole-dithiocarbamate based, selective LSD1 inactivators inhibit gastric cancer cell growth, invasion and migration
authors: Yi-Chao Zheng; Ying-Chao Duan; ...; Hong-Min Liu
journal: Journal of Medicinal Chemistry
date: 2014-01-06
pmid: 24131029
pmcid: 3881423
cited_by_count: 187
techniques: Activity Assay; Dilution Assay; Immunoprecipitation; In Vitro; Methylation; Microscale Thermophoresis; Purification; Staining; Western Blot
bioz_score: 94
title: Triazole–Dithiocarbamate Based Selective Lysine Specific Demethylase 1 (LSD1) Inactivators Inhibit Gastric Cancer Cell Growth, Invasion, and Migration
authors: Yi-Chao Zheng; Ying-Chao Duan; ...; Hong-Min Liu
journal: Journal of Medicinal Chemistry
date: 2013-11-01
doi: 10.1021/jm401002r
cited_by_count: 0
techniques: Cell Culture; Purification; Staining; Western Blot
bioz_score: 94
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