Recombinant Human HSP27 protein (Catalog#10351-H08E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1-Lys 205) .
Source
Monoclonal Rabbit IgG Clone #017
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
HSP27 was immunoprecipitated using: Lane A:0.5 mg Caco-2 Whole Cell Lysate 2 µL anti-HSP27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-HSP27 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size: 25 kDa
HSP27 was immunoprecipitated using: Lane A:0.5 mg Caco-2 Whole Cell Lysate 2 µL anti-HSP27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-HSP27 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size: 25 kDa