| Catalog No. | Size | Price (USD) |
| 11996-MM34-F-25 | 25 Tests | $103.68 |
| 11996-MM34-F-100 | 100 Tests | $222.17 |
General Information
| Product name | Anti-c-Kit Antibody (FITC), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human c-Kit |
| Immunogen | Recombinant Human c-Kit Protein (Catalog#11996-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human c-Kit (rh c-Kit; Catalog#11996-H08H; P10721-2; Met1-Thr516) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| Source | Monoclonal Mouse IgG1 Clone #34 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Concentration | 5 μl/Test, 0.1 mg/ml |
| Conjugate | FITC |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! |
Synonyms: Anti-C-Kit Antibody; Anti-CD117 Antibody; Anti-MASTC Antibody; Anti-PBT Antibody; Anti-SCFR Antibody
Image / Experimental Information
| Image 1 Description | Flow cytometric analysis of Human KIT(CD117) expression on HEL92 cells. Cells were stained with FITC-conjugated anti-Human KIT(CD117). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Image 1 Alt Text | Human c-Kit Flow Cytometry (FC) 23292 |
| Image 1 URL | Source image |
Background Information
| Full Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Description | C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients. |
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