| Catalog No. | Size | Price (USD) |
| 10619-T52-50 | 50 µL | $147.96 |
| 10619-T52-100 | 100 µL | $251.64 |
| 10619-T52-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-HtrA2/Omi Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human HtrA2/Omi |
| Immunogen | Recombinant Human HtrA2 Protein (Catalog#10619-H08E) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human HtrA2 (rh HtrA2; Catalog#10619-H08E; O43464-1; Ala134-Glu458). HtrA2 specific IgG was purified by Human HtrA2 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-MGCA8 Antibody; Anti-OMI Antibody; Anti-PARK13 Antibody; Anti-PRSS25 Antibody
Image / Experimental Information
| Image 1 Description | HTRA2 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg HL-60 Whole Cell Lysate 1 µL anti-HTRA2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-HTRA2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDa |
| Image 1 Alt Text | Human HtrA2 Immunoprecipitation(IP) 6167 |
| Image 1 URL | Source image |
| Image 2 Description | HTRA2 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg HL-60 Whole Cell Lysate 1 µL anti-HTRA2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-HTRA2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size: 37 kDa |
| Image 2 Alt Text | Human HtrA2 Western blot (WB) 6951 |
| Image 2 URL | Source image |
Background Information
| Full Name | HtrA serine peptidase 2 |
| Description | Serine protease HTRA2, also known as high-temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein that belongs to the peptidase S1B family. HTRA2 contains one PDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity or by a BIRC inhibition-independent, caspase-independent, and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity, and postural instability, as well as by a clinically significant response to treatment with levodopa. |
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