| Catalog No. | Size | Price (USD) |
| 10702-T16-20 | 20 µL | $74.06 |
| 10702-T16-50 | 50 µL | $103.68 |
| 10702-T16-100 | 100 µL | $148.11 |
General Information
| Product name | Rabbit Anti-Human IgG-Fc Secondary Antibody, Rabbit PAb, Antigen Affinity Purified |
| Validated applications | ELISA (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | By ELISA this antibody reacts specifically with Human IgG-Fc. May has cross-reactivity with the immunoglobulins of other species. |
| Immunogen | Recombinant Human IgG1-Fc Protein (Catalog#10702-HNAH) |
| Preparation | Produced in rabbits immunized with recombinant human IgG1-Fc (Catalog#10702-HNAH; P01857-1; Glu99-Lys330,C103S). This polyclonal antibody was purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. |
| References |
|
title: Development and Qualification of a Nipah Virus Glycoprotein-Specific IgG ELISA for the Assessment of Human Antibody Responses.
authors: Mohammad Mamun Alam; Tahsin Tabassum Anonto; ...; Mohammed Ziaur Rahman
journal: Vaccines
date: 2026-06-25
pmid: 42347655
doi: 10.3390/vaccines14060534
cited_by_count: 0
techniques: Affinity Purification; Enzyme-linked Immunosorbent Assay
bioz_score: 94
title: First-in-class small molecule inhibitors of ICOS/ICOSL interaction as a novel class of immunomodulators
authors: Somaya A. Abdel-Rahman; Katarzyna Świderek; Moustafa T. Gabr
journal: RSC Medicinal Chemistry
date: 2023-09-19
pmcid: 10507805
cited_by_count: 0
bioz_score: 94
title: Computational Microscopy of Glycoproteins: from Viruses to T Cells
authors: Rosenfeld, Mia Aurora
date: 2023-01-01
cited_by_count: 0
techniques: Modification
bioz_score: 94
