Produced in rabbits immunized with E. coli-derived Human C10orf4 / FRA10AC1 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Anti-C10orf4/FRA10AC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: NIH/3T3 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:37 kDa Observed band size:40 kDa
Anti-C10orf4/FRA10AC1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: NIH/3T3 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:37 kDa Observed band size:40 kDa
Image 2 Alt Text
Human C10orf4/FRA10AC1 Immunoprecipitation(IP) 34785
The FRA10AC1 gene contains a tandem CGG repeat region within a CpG island that normally consists of 8-14 repeats but can expand to over 200 repeats. The major FRA10AC1 transcript encodes a deduced 315-amino acid protein with a calculated molecular mass of 37.5 kD. FRA10AC1 contains several sites for serine phosphorylation and a bipartite nuclear localization signal embedded within a larger lysine-rich region. The FRA10AC1 protein is conserved between species. The human protein shares 86% identity with mouse Fra10ac1 and about 35% identity with plant and C. elegans Fra10ac1. A central sequence of FRA10AC1 is most highly conserved. The FRA10AC1 gene is found within the rare FRA10A folate-sensitive fragile site.
References
Sarafidou T, et al. (2004) Folate-sensitive fragile site fra10a is due to an expansion of a cgg repeat in a novel gene, fra10ac1, encoding a nuclear protein. Genomics 84 (1): 69-81.
De Leon-Luis J, et al. (2005) Prenatal diagnosis of fra10a: A case report and literature review. Am J Med Genet A 136 (1): 63-65.