WB (FAQ Protocol), IHC-P (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human C1orf109
Immunogen
E. coli-derived Human C1orf109 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human C1orf109 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
Anti-C1orf109 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: RPMI-8226 Whole Cell Lysate Lane C: U251MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:26 kDa (We are unsure as to the identity of these extra bands.)
Anti-C1orf109 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: RPMI-8226 Whole Cell Lysate Lane C: U251MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:26 kDa (We are unsure as to the identity of these extra bands.)
Anti-C1orf109 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lane B: RPMI-8226 Whole Cell Lysate Lane C: U251MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:26 kDa (We are unsure as to the identity of these extra bands.)
Homo Sapiens Chromosome 1 ORF109 (c1orf109, GenBank ID: NM_017850.1), which encodes a substrate of CK2. C1orf109 gene is located on 1p34.3. The function of the gene has been poorly studied. Several research groups identified that C1orf109 dysregulation might involve in the developmental process or cause diseases such as tumors. Deletion of 1p34.3 locus, which includes C1orf109, could cause facial dysmorphism. The promoter region of C1orf109 gene was modified by methylation in aging and some diseases, such as keloids and systemic lupus erythematosus. C1ORF109 may be the downstream target of protein kinase CK2 and involved in the regulation of cancer cell proliferation.
References
Liu SS, et al. (2012) Identification and characterization of a novel gene, c1orf109, encoding a ck2 substrate that is involved in cancer cell proliferation. J Biomed Sci 19 (1): 49.
Dou P, et al. (2020) C1orf109l binding dhx9 promotes DNA damage depended on the r-loop accumulation and enhances camptothecin chemosensitivity. Cell Prolif 53 (9): e12875.
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