| Catalog No. | Size | Price (USD) |
| 10222-RP01-100 | 100 µL | $147.96 |
| 10222-RP01-200 | 200 µL | $251.64 |
| 10222-RP01-400 | 400 µL | $355.32 |
General Information
| Product name | Anti-Lipocalin-2/NGAL Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Lipocalin-2/NGAL |
| Immunogen | Recombinant Human LCN2 protein (Catalog#10222-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human Lipocalin-2 (rh Lipocalin-2; Catalog#10222-H08H; NP_005555.2; Met 1-Gly 198). Total IgG was purified by Protein A affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-24p3 Antibody; Anti-HNL Antibody; Anti-MSFI Antibody; Anti-NGAL Antibody; Anti-p25 Antibody
Image / Experimental Information
| Image 1 Description | Anti-LCN2 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:27 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Human NGAL/LCN2/Lipocalin-2 Western blot (WB) 13149 |
| Image 1 URL | Source image |
Background Information
| Full Name | lipocalin 2 |
| Description | Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25 kDa protein belonging to the lipocalin superfamily. It was initially found in activated neutrophils, however, many other cells, like kidney tubular cells, may produce NGAL in response to various insults. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI). Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein that specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute-phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect, as has already been observed in different tumors (e.g. breast, stomach, esophagus, brain) in humans. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival, and morphogenesis. |
| References |
|
title: Empowering an Acute Kidney Injury 3D Graphene-Based Sensor Using Extreme Learning Machine
authors: Netnapa Sittihakote; Pobporn Danvirutai; ...; Chavis Srichan
journal: ACS Omega
date: 2024-05-01
doi: 10.1021/acsomega.4c01315
pmcid: 11097169
cited_by_count: 0
techniques: Biomarker Assay; Comparison; Concentration Assay; Enzyme-linked Immunosorbent Assay; Modification; Transduction
bioz_score: 92
