| Catalog No. | Size | Price (USD) |
| 101351-T38-50 | 50 µL | $147.96 |
| 101351-T38-100 | 100 µL | $251.64 |
| 101351-T38-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-MEK1 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Mouse, Rat (Species predicted to react based on 100% sequence homology) |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human MEK1/MAP2K1/MKK1 |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MEK1/MAP2K1/MKK1, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CFC3 Antibody; Anti-MAPKK1 Antibody; Anti-MEK1 Antibody; Anti-MEL Antibody; Anti-MKK1 Antibody; Anti-PRKMK1 Antibody
Image / Experimental Information
| Image 1 Description | Anti-MAP2K1 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa |
| Image 1 Alt Text | Human MEK1/MAP2K1/MKK1 Western blot (WB) 17268 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-MAP2K1 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa |
| Image 2 Alt Text | Human MEK1/MAP2K1/MKK1 Immunofluorescence(IF) 17269 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-MAP2K1 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa |
| Image 3 Alt Text | Human MEK1/MAP2K1/MKK1 Immunoprecipitation(IP) 17270 |
| Image 3 URL | Source image |
Background Information
| Full Name | mitogen-activated protein kinase kinase 1 |
| Description | MEK1, also known as MAP2K1 and MKK1, is a member of the dual-specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in the brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation, and development. Binding extracellular ligands such as growth factors, cytokines, and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause Cardiofaciocutaneous Syndrome. |
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