WB (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human CALD1/caldesmon
Immunogen
E. coli-derived Human CALD1/caldesmon fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human CALD1/caldesmon fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Anti-CALD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:75 kDa
Anti-CALD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:75 kDa
Image 2 Alt Text
Human CALD1/caldesmon Immunofluorescence(IF) 28747
Anti-CALD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:93 kDa Observed band size:75 kDa
Image 3 Alt Text
Human CALD1/caldesmon Immunoprecipitation(IP) 28748