| Catalog No. | Size | Price (USD) |
| 10082-MM05-20 | 20 µL | $88.87 |
| 10082-MM05-50 | 50 µL | $147.96 |
| 10082-MM05-100 | 100 µL | $251.64 |
| 10082-MM05-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-MMP-2 Antibody, Mouse Monoclonal |
| Validated applications | ELISA (FAQ Protocol), FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human MMP-2 No cross-reactivity in ELISA with Human MMP1 Human MMP7 Human MMP9 Human cell lysate (293 cell line) |
| Immunogen | Recombinant Human MMP-2 protein (Catalog#10082-HNAH) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MMP-2 (rh MMP-2; Catalog#10082-HNAH; NP_004521.1; Met 1-Cys660). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG2a Clone #05 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CLG4 Antibody; Anti-CLG4A Antibody; Anti-MMP-2 Antibody; Anti-MMP-II Antibody; Anti-MONA Antibody; Anti-TBE-1 Antibody
Image / Experimental Information
| Image 1 Description | Flow cytometric analysis of Human MMP2 expression on MG63 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human MMP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Image 1 Alt Text | Human MMP-2 Flow Cytometry (FC) 21282 |
| Image 1 URL | Source image |
Background Information
| Full Name | matrix metallopeptidase 2 |
| Description | Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN), Her2/neu, growth factors, cytokines, and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to a favorable prognosis in patients with a hormone receptor-negative tumor, usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity, MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems, including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment. |
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