WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Canine
Specificity
Canine Carbonic Anhydrase IX/CA9
Immunogen
Recombinant Canine Carbonic Anhydrase IX / CA9 protein (Catalog#70028-D08H)
Preparation
Produced in rabbits immunized with purified, recombinant Canine Carbonic Anhydrase IX / CA9 (Catalog#70028-D08H; NP_001138646.1; Met1-Leu410). Carbonic Anhydrase IX / CA9 specific IgG was purified by Canine Carbonic Anhydrase IX / CA9 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
canine CA9 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 0.5 µL anti-canine CA9 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-canine CA9 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa Observed band size: 55 kDa
canine CA9 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate 0.5 µL anti-canine CA9 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-canine CA9 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 55 kDa Observed band size: 55 kDa
Image 2 Alt Text
Canine Carbonic Anhydrase IX/CA9 Western blot (WB) 6801
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
Research Areas
Cancer Drug Targets
Tags
Cancer Immunotherapy
Immune Checkpoint
Immunotherapy
Targeted Therapy
References
Loncaster JA, et al. (2001) Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia: correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix. Cancer Res. 61(17): 6394-9.
Zvada J, et al. (2003) Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients. Br J Cancer. 89(6): 1067-71.
Pan P, et al. (2006) Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 571(Pt 2): 319-27.
Zhou GX, et al. (2010) Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology. 75(2): 257-61.
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