| Catalog No. | Size | Price (USD) |
| 310803-T32-50 | 50 µL | $147.96 |
| 310803-T32-100 | 100 µL | $251.64 |
| 310803-T32-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-HUWE1 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human HUWE1 |
| Immunogen | E. coli-derived Human HUWE1 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human HUWE1 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ARF-BP1 Antibody; Anti-HECTH9 Antibody; Anti-HSPC272 Antibody; Anti-Ib772 Antibody; Anti-KIAA0312 Antibody; Anti-KIAA1578 Antibody; Anti-LASU1 Antibody; Anti-MRXST Antibody; Anti-MULE Antibody; Anti-URE-B1 Antibody; Anti-UREB1 Antibody
Image / Experimental Information
| Image 1 Description | Anti-HUWE1 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:481 kDa |
| Image 1 Alt Text | Human HUWE1 Western blot (WB) 35196 |
| Image 1 URL | Source image |
Background Information
| Description | HUWE1 (HECT, UBA And WWE Domain Containing E3 Ubiquitin Protein Ligase 1) is a large proteins. There are conflicting reports of the role of HUWE1 in regulating the oncogene c-MYC (MYC). In one model, HUWE1 regulates the stability of MYC through K48-linked ubiquitination and knockdown of HUWE1 leads to increased MYC and drives tumor progression [16]. Conversely, in other tumor types HUWE1 activates transcription of MYC through K63-linked ubiquitination and HUWE1 silencing or inhibition prevents MYC signaling and reduces tumor cell proliferation. HUWE1 may play a cell-type and context-dependent role in MYC regulation. |
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