| Catalog No. | Size | Price (USD) |
| 68045-R118-H-20 | 20 µg | $88.87 |
| 68045-R118-H-50 | 50 µg | $177.73 |
| 68045-R118-H-100 | 100 µg | $296.22 |
General Information
| Product name | Rabbit Anti-Mouse IgM / Immunoglobulin M Secondary Antibody (HRP), Rabbit MAb |
| Validated applications | ELISA (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse IgM |
| Immunogen | Purified Mouse IgM Protein |
| Preparation | This antibody was obtained from a rabbit immunized with purified mouse IgM protein, and conjugated with horseradish-peroxidase (HRP). |
| Source | Monoclonal Rabbit IgG Clone #118 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | HRP |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-Igh-6 Antibody; Anti-Igh-M Antibody; Anti-Igh6 Antibody; Anti-Ighm Antibody; Anti-Igm Antibody; Anti-muH Antibody
Image / Experimental Information
| Image 1 Description | ELISA of immunoglobulins shows Recombinant Anti-IgM Antibody (HRP), Rabbit Monoclonal (Cat# 68045-R118-H) reacts to mouse IgM. No cross reactivity with mouse IgG1, mouse IgG2a, mouse IgG2b, mouse IgG3, mouse IgA, human IgG. The plate was coated with 1 μg/mL of different immunoglobulins. 0.4 μg/mL of Recombinant Anti-IgM Antibody (HRP), Rabbit Monoclonal (Cat# 68045-R118-H) was used as the secondary antibody. (Validation Experiment) |
| Image 1 Alt Text | Mouse IgM Enzyme linked immunosorbent assay (ELISA) 43047 |
| Image 1 URL | Source image |
Background Information
| Description | Antibodies, also known as Immunoglobulins ( Ig ), are gamma globulin proteins that are found in blood or other bodily fluids of vertebrates and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. Antibodies can come in different varieties known as isotypes or classes. In placental mammals, there are five antibody isotypes known as IgA, IgD, IgE, IgG, and IgM. They are each named with an " Ig " prefix that stands for immunoglobulin, another name for antibody, and differ in their biological properties, functional locations, and ability to deal with different antigens. IgM is expressed on the surface of B cells and in a secreted form with very high avidity. It eliminates pathogens in the early stages of B cell-mediated immunity before there is sufficient IgG. IgM is by far the physically largest antibody in the human circulatory system. It is produced after an animal has been exposed to an antigen for an extended time or when an animal is exposed to an antigen for the second time. IgM forms polymers where multiple immunoglobulins are covalently linked together with disulfide bonds, mostly as a pentamer but also as a hexamer. Because each monomer has two antigen-binding sites, a pentameric IgM has 1 binding site. Typically, however, IgM cannot bind 1 antigen at the same time because the large size of most antigens hinders binding to nearby sites. Due to its polymeric nature, IgM possesses high avidity and is particularly effective at complement activation. |
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