| Catalog No. | Size | Price (USD) |
| 50350-T44-50 | 50 µL | $147.96 |
| 50350-T44-100 | 100 µL | $251.64 |
| 50350-T44-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Carbonic Anhydrase IV/CA4 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse Carbonic Anhydrase IV/CA4 |
| Immunogen | Recombinant Mouse Car4 Protein (Catalog#50350-M08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Mouse Car4 (rM Car4; Catalog#50350-M08H; NP_031633.1; Met1-Ser277). Car4 specific IgG was purified by Mouse Car4 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-Ca4 Antibody; Anti-Car4 Antibody
Image / Experimental Information
| Image 1 Description | Anti-Carbonic Anhydrase IV rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse lung tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Mouse Carbonic Anhydrase IV Western blot (WB) 32123 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-Carbonic Anhydrase IV rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse lung tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.) |
| Image 2 Alt Text | Mouse Carbonic Anhydrase IV Immunohistochemistry(IHC) 32124 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-Carbonic Anhydrase IV rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse lung tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.) |
| Image 3 Alt Text | Mouse Carbonic Anhydrase IV Immunohistochemistry(IHC) 32125 |
| Image 3 URL | Source image |
| Image 4 Description | Anti-Carbonic Anhydrase IV rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse lung tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:37 kDa (We are unsure as to the identity of these extra bands.) |
| Image 4 Alt Text | Mouse Carbonic Anhydrase IV Immunoprecipitation(IP) 32126 |
| Image 4 URL | Source image |
Background Information
| Full Name | carbonic anhydrase IV |
| Description | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3 dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII. |
| References |
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