Produced in rabbits immunized with E. coli-derived Human LUC7L2 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Immunofluorescence staining of LUC7L2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LUC7L2 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
LUC7L2 (also known as CGI-59, CGI-74 and LUC7B2) resides on chromosome of 7q34. Indeed, chromosome 7 represents one of the most frequently altered chromosomes in patients with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS), with a variety of deletions and translocations described. LUC7L2 protein contains a C2H2-type zinc finger, coiled-coil region and arginine, serine-rich (RS) domain. LUC7L2 transcripts are widely distributed in mammalian tissues, and undergo alternative splicing and polyadenylation. LUC7L2 is also co-localized with U1-70K and may function with SCNM1 in recognition of weak splice donor sites.
References
Singh H, et al. (2013) Putative rna-splicing gene luc7l2 on 7q34 represents a candidate gene in pathogenesis of myeloid malignancies. Blood Cancer J 3 (5): e117.
Howell VM, et al. (2007) Evidence for a direct role of the disease modifier scnm1 in splicing. Hum Mol Genet 16 (20): 2506-2516.