| Catalog No. | Size | Price (USD) |
| 50531-RP02-50 | 50 µL | $147.96 |
| 50531-RP02-100 | 100 µL | $251.64 |
| 50531-RP02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-15-PGDH Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse 15-PGDH |
| Immunogen | Recombinant Mouse HPGD / 15-PGDH protein (Catalog#50531-M08E) |
| Preparation | Produced in rabbits immunized with purified, recombinant Mouse HPGD / 15-PGDH (rM HPGD / 15-PGDH; Catalog#50531-M08E; Q8VCC1; Met 1-Ser 269). HPGD / 15-PGDH specific IgG was purified by Mouse HPGD / 15-PGDH affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-15-PGDH Antibody; Anti-Hpgd Antibody; Anti-Pgdh1 Antibody
Image / Experimental Information
| Image 1 Description | Mouse HPGD was immunoprecipitated using: Lane A:0.5 mg Caco-2 Whole Cell Lysate 2 µL anti-Mouse HPGD rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-Mouse HPGD rabbit polyclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size: 29 kDa |
| Image 1 Alt Text | Mouse HPGD/15-PGDH Immunoprecipitation(IP) 8241 |
| Image 1 URL | Source image |
| Image 2 Description | Mouse HPGD was immunoprecipitated using: Lane A:0.5 mg Caco-2 Whole Cell Lysate 2 µL anti-Mouse HPGD rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-Mouse HPGD rabbit polyclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size: 29 kDa |
| Image 2 Alt Text | Mouse HPGD/15-PGDH Western blot (WB) 13685 |
| Image 2 URL | Source image |
Background Information
| Full Name | hydroxyprostaglandin dehydrogenase 15-(NAD) |
| Description | 15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone, and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoarthropathy autosomal recessive (PHOAR), cranio-osteoarthropathy (COA), and isolated congenital nail clubbing. |
| References |
|
title: miR-620 promotes tumor radioresistance by targeting 15-hydroxyprostaglandin dehydrogenase (HPGD).
authors: Xiaoyong Huang; Samira Taeb; ...; Stanley K Liu
journal: Oncotarget
date: 2016-09-27
pmid: 26068950
doi: 10.18632/oncotarget.4210
cited_by_count: 22
techniques: Western Blot
bioz_score: 90
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