Anti-CD25/IL-2R alpha Antibody, Mouse Monoclonal sinobiological 90265-MM07
$243.00
In stock
SKU
90265-MM07
| Catalog No. | Size | Price (USD) |
| 90265-MM07-100 | 100 µL | $251.64 |
General Information
| Product name | Anti-CD25/IL-2R alpha Antibody, Mouse Monoclonal |
| Validated applications | ELISA (FAQ Protocol) |
| Species reactivity | Reacts with: Cynomolgus |
| Specificity | Cynomolgus CD25/IL-2R alpha |
| Immunogen | Recombinant Cynomolgus CD25/IL-2R alpha Protein (Catalog# 90265-C08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus CD25/IL-2R alpha (Catalog#90265-C08H; H6WS54; Met1-Arg213). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #07 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Full Name | interleukin 2 receptor, alpha |
| Description | CD25 (alpha-chain of the IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high-affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covalently with the IL2 receptor beta and gamma chain, and subsequently initiates the intracellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a piece of strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development are associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of SIL-2Ralpha. In most hematological malignancies, including different types of leukemias and lymphomas, SIL-2Ralpha is released directly from the surface of neoplastic cells thus reflecting the tumor bulk, turnover, and activity. Several studies have proved that not only lymphoid cancer cells but also some non-lymphoid cancer cells, express IL-2R on their surface. They include malignant melanoma and carcinomas of the kidney, head and neck, esophagus, and lung. Thus, sIL-2Ralpha is elevated in most proliferative disturbances of the hematopoietic system and many solid tumors. |
| Research Areas |
|
| Related Pathways |
|
| Tags |
|
| References |
|
title: IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells
authors: Victoria A. Wong; Kristie N. Dinh; ...; Lucile E. Wrenshall
journal: Frontiers in Immunology
date: 2024-05-15
doi: 10.3389/fimmu.2024.1369818
pmcid: 11133634
cited_by_count: 0
techniques: Adsorption; Blocking Assay; Cell Culture; Co-Culture Assay; Cytometry; Derivative Assay; Expressing; Flow Cytometry; Fluorescence; Imaging; Immunodetection; Immunoprecipitation; Isolation; Labeling; Membrane; Microscopy; Purification; SDS Page; Sequencing; Software; Staining; Western Blot
bioz_score: 92
Reviews
Write Your Own Review