Produced in rabbits immunized with E. coli-derived Human CD2BP2 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Anti-CD2BP2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.)
Anti-CD2BP2 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: U-251MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:38 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.)
The adaptor protein CD2BP2, originally identified as a binding partner of the adhesion molecule CD2, is a pre-spliceosomal assembly factor that utilizes its glycine-tyrosine-phenylalanine (GYF) domain to co-localize with spliceosomal proteins. CD2BP2 confers binding to proline-rich sequences (PRS) via its GYF domain. CD2BP2 plays a crucial role in CD2-triggered T cell activation and nuclear splicing. CD2BP2 is a non-redundant splicing factor that is essential for embryonic development and podocyte integrity.
References
Kofler M, et al. (2004) Recognition sequences for the gyf domain reveal a possible spliceosomal function of cd2bp2. J Biol Chem 279 (27): 28292-28297.
Heinze M, et al. (2007) Investigating the functional role of cd2bp2 in t cells. Int Immunol 19 (11): 1313-1318.
Albert GI, et al. (2015) The gyf domain protein cd2bp2 is critical for embryogenesis and podocyte function. J Mol Cell Biol 7 (5): 402-414.