| Catalog No. | Size | Price (USD) |
| 13142-MM06-P-25 | 25 Tests | $222.17 |
| 13142-MM06-P-100 | 100 Tests | $414.71 |
General Information
| Product name | Anti-CD3 zeta/CD247 Antibody (PE), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD3 zeta/CD247 |
| Immunogen | Recombinant Human CD3 zeta / CD247 Protein (Catalog#13142-H09E) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3 zeta / CD247 (rh CD3 zeta / CD247; Catalog#13142-H09E; P20963-1; Arg52-Arg164) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Source | Monoclonal Mouse IgG2a Clone #06 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Concentration | 5 μl/Test, 0.1 mg/ml |
| Conjugate | PE |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! |
Synonyms: Anti-CD3-ZETA Antibody; Anti-CD3H Antibody; Anti-CD3Q Antibody; Anti-CD3Z Antibody; Anti-CD3ZETA Antibody; Anti-IMD25 Antibody; Anti-T3Z Antibody; Anti-TCRZ Antibody
Image / Experimental Information
| Image 1 Description | Flow cytometric analysis of Human CD3 Zeta expression on human whole blood lymphocytes.The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with PE-conjugated anti-Human CD3 Zetaand and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.. |
| Image 1 Alt Text | Human CD3 zeta/CD247 Flow Cytometry (FC) 35889 |
| Image 1 URL | Source image |
Background Information
| Full Name | CD247 molecule |
| Description | CD247, also known as CD3-ZETA, belongs to the CD3Z/FCER1G family. It contains 3 ITAM domains. As a -cell receptor zeta, CD247 forms the T-cell receptor-CD3 complex together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and –epsilon. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247 gene. Defects in CD247 can cause immunodeficiency due to defect in CD3-zeta. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. CD247 may play a role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. |
| References |
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