Anti-CD3 zeta/CD247 Antibody, Rabbit Polyclonal sinobiological 200144-T46
$243.00
In stock
SKU
200144-T46
| Catalog No. | Size | Price (USD) |
| 200144-T46-100 | 100 µL | $251.64 |
General Information
| Product name | Anti-CD3 zeta/CD247 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human CD3 zeta/CD247 |
| Immunogen | E. coli-derived Human CD3 zeta/CD247 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human CD3 zeta/CD247 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CD3-ZETA Antibody; Anti-CD3H Antibody; Anti-CD3Q Antibody; Anti-CD3Z Antibody; Anti-CD3ZETA Antibody; Anti-IMD25 Antibody; Anti-T3Z Antibody; Anti-TCRZ Antibody
Image / Experimental Information
| Image 1 Description | Anti-CD3 zeta rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa (We are unsure as to the identity of these extra bands.) |
| Image 1 Alt Text | Human CD3 zeta/CD247 Western blot (WB) 38703 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-CD3 zeta rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa (We are unsure as to the identity of these extra bands.) |
| Image 2 Alt Text | Human CD3 zeta/CD247 Immunohistochemistry(IHC) 38704 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-CD3 zeta rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa (We are unsure as to the identity of these extra bands.) |
| Image 3 Alt Text | Human CD3 zeta/CD247 Immunohistochemistry(IHC) 38705 |
| Image 3 URL | Source image |
| Image 4 Description | Anti-CD3 zeta rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa (We are unsure as to the identity of these extra bands.) |
| Image 4 Alt Text | Human CD3 zeta/CD247 Immunofluorescence(IF) 38706 |
| Image 4 URL | Source image |
| Image 5 Description | Anti-CD3 zeta rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HEK293 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:18 kDa Observed band size:18 kDa (We are unsure as to the identity of these extra bands.) |
| Image 5 Alt Text | Human CD3 zeta/CD247 Immunoprecipitation(IP) 38707 |
| Image 5 URL | Source image |
Background Information
| Full Name | CD247 molecule |
| Description | CD247, also known as CD3-ZETA, belongs to the CD3Z/FCER1G family. It contains 3 ITAM domains. As a -cell receptor zeta, CD247 forms the T-cell receptor-CD3 complex together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and –epsilon. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247 gene. Defects in CD247 can cause immunodeficiency due to defect in CD3-zeta. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. CD247 may play a role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. |
| References |
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