TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal ) transgen AT341-01
$50.00
In stock
SKU
AT341-01
Introduction and Features
The kit provides all the necessary components (TransScript® RT, RNase Inhibitor, Anchored Oligo(dT)18 Primer, Random Primer (N9), dNTPs, Buffer) for reverse transcription reaction. It is provided at 5× concentration and used by adding gDNA remover, RNA template and H2O for efficient first-strand cDNA synthesis. Simultaneously, residual genomic DNA from RNA template can be removed. 5×TransScript® All-in-One No-RT Control SuperMix for qPCR is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the template for the qPCR reaction is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow.The resulting cDNA is suitable for qPCR, not for regular PCR.
Features
- “All SuperMix” form: Simultaneous cDNA synthesis and genomic DNA removal by only adding gDNA remover, RNA template and H2O.
- High synthesis efficiency enabled by optimal ratio of Oligo(dT)18 Primer to Random Primer (N9) and optimized composition of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and high synthesis efficiency for short cDNA.
- Only 15 minutes for reverse transcription.
- High compatibility with qPCR reagents.
| Applications | High copy and low copy gene detection. |
| Storage | at -18°C or below for two years |
| Shipping | Dry ice (-70 ℃) |
Product Composition
| Component | AT341-01 (50 rxns ) | AT341-02 (100 rxns ) | AT341-03 (500 rxns ) |
| 5×TransScript® All-in-One SuperMix for qPCR | 200 μl | 400 μl | 5×400 μl |
| 5×TransScript® All-in-One No-RT Control SuperMix for qPCR | 20 μl | 40 μl | 200 μl |
| gDNA Remover | 50 μl | 100 μl | 5×100 μl |
| RNase-free Water | 1 ml | 2×1 ml | 2×5 ml |
References
1 Sun X, Zhang T, Tong B, et al. POGZ suppresses 2C transcriptional program and retrotransposable elements[J]. Cell Reports, 2023.(IF 8.80)
2 Yuan F, Cai J, Wu J, et al. Z-DNA binding protein 1 promotes heatstroke-induced cell death[J]. Science, 2022.(IF 47.72)
3 Yuan F, Cai J, Wu J, et al. Z-DNA binding protein 1 promotes heatstroke-induced cell death[J]. Science, 2022.(IF 63.71)
4 Yu Z, Deng P, Chen Y, et al. Inhibition of the PLK1‐coupled cell cycle machinery overcomes resistance to oxaliplatin in colorectal cancer[J]. Advanced Science, 2021.(IF 16.80)
5 Sun X, Peng X, Cao Y, et al. ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling[J]. Nature communications, 2020.(IF 12.12)
6 Wu H, Qu X, Dong Z, et al. WUSCHEL triggers innate antiviral immunity in plant stem cells[J]. Science, 2020.(IF 41.84)
7 Ji S, Luo Y, Cai Q, et al. LC domain-mediated coalescence is essential for Otu enzymatic activity to extend Drosophila lifespan[J]. Molecular cell, 2019.(IF 14.20)
Reviews
Write Your Own Review