TransScript® Uni All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal) transgen AU341-02
$50.00
In stock
SKU
AU341-02
Introduction and Features
The kit provides all the necessary reagents for reverse transcription reaction (including TransScript® Uni RT, RNase Inhibitor, Anchored Oligo (dT)20 Primer, Random Primer (N9), dNTPs, Buffer). During the reaction, simply add the 5× All-in-One Reaction Mix for qPCR, TransScript® Uni All-in-One Enzyme Mix, RNA template, and H2O. Under conditions ranging from 42°C to 65°C, the system enables efficient first-strand cDNA synthesis while simultaneously removing residual genomic DNA from the RNA template. 20×No RT Control Mix is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the qPCR template is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow. The resulting cDNA is only suitable for qPCR, not for regular PCR.
Features
- Board range reaction temperature (42°C-65°C).
- All SuperMix form: Simultaneous cDNA synthesis and genomic DNA removal by only adding the 5× All-in-One Reaction Mix
for qPCR, TransScript® UniAll-in-One Enzyme Mix, RNA template, and H2O.
- High synthesis efficiency enabled by optimal ratio of Oligo(dT)20 Primer to Random Primer (N9) and optimized composition
of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and specifically
high synthesis efficiency for short cDNA.
- Only 5 minutes for reverse transcription.
- High compatibility with qPCR reagents.
| Applications | High-copy number and low-copy number gene detection |
| Storage | at -18℃ or below for two years. |
| Shipping | Dry ice (-70 ℃) |
Product Composition
| Component | AU341-02 (100 rxns) |
| 5×All-in-One Reaction Mix for qPCR | 400 μl |
| TransScript® Uni All-in-One Enzyme Mix | 100 μl |
| 20×No RT Control Mix | 40 μl |
| RNase-free Water | 2×1 ml |
References
1 Qin T, Zhang G, Zheng Y, et al. A population of stem cells with strong regenerative potential discovered in deer antlers[J]. Science, 2023.(IF 63.71)
2 Yang L, Wu X, Liu S, et al. Comprehensive Analysis of BrHMPs Reveals Potential Roles in Abiotic Stress Tolerance and Pollen–Stigma Interaction in Brassica rapa[J]. Cells, 2023.(IF 7.66)
3 Yang X, Liu S, Lu W, et al. Delta and jagged are candidate target genes of RNAi biopesticides for the control of Nilaparvata lugens[J]. Frontiers in Bioengineering and Biotechnology, 2022.(IF 6.064)
4 Yuan Y, Fu D, Xu Y, et al. Pt (IV) Prodrug as a Potential Antitumor Agent with APE1 Inhibitory Activity[J]. Journal of Medicinal Chemistry, 2022.(IF 8.039)
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