pEASY®-T1 Simple Cloning Kit transgen CT111-01
$50.00
In stock
SKU
CT111-01
Introduction and Features
pEASY®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 minutes fast ligation of Taq-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •SR primer and M13 forward primer for sequencing. •T7 promoter for in vitro transcription. •Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
| Storage | Competent Cell at -70°C for six months; others at -20°C for 9 months |
| Shipping | Dry ice (-70℃) |
Product Composition
| Component | CT111-01 (20 rxns) | CT111-02 (60 rxns) |
| pEASY ®-T1 Simple Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| SR Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
References
1 Liu W, Li F, Ma J, et al. Scallop interferon regulatory factor 1 interacts with myeloid differentiation primary response protein 88 and is crucial for antiviral innate immunity[J]. International Journal of Biological Macromolecules, 2022.(IF 8.02)
2 Niu X L, Li H L, Li R, et al. Transcription factor SlBEL2 interferes with GOLDEN2‐LIKE and influences green shoulder formation in tomato fruits[J]. The Plant Journal, 2022.(IF 7.09)
3 Liu X, Xu B, Yang J, et al. UHRF2 commissions the completion of DNA demethylation through allosteric activation by 5hmC and K33-linked ubiquitination of XRCC1[J]. Molecular cell, 2021.(IF 17.97)
4 Chen H H, He Y J, Liang M H, et al. The expression pattern of β‐carotene ketolase gene restricts the accumulation of astaxanthin in Dunaliella under salt stress[J]. Journal of Cellular Physiology, 2022.(IF 6.38)
5 Zeng Y H, Cai Z H, Zhu J M, et al. Two hierarchical LuxR-LuxI type quorum sensing systems in Novosphingobium activate microcystin degradation through transcriptional regulation of the mlr pathway[J]. Water Research, 2020.(IF 9.13)
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