pEASY®-Blunt Simple Cloning Kit transgen CB111-01
$50.00
In stock
SKU
CB111-01
Introduction and Features
pEASY ®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY ® -Blunt Cloning Vector. The vector contains the
LacZ gene, which allows for blue-white screening on plates supplemented with IPTG and X-gal. It is suitable for blunt-end cloning.
| Storage | Trans1-T1 Phage Resistant Chemically Competent Cell at -70oC or below for one year; others at -18℃ or below for one year |
| Shipping | Dry ice (-70℃) |
Product Composition
| Component | CB111-01 (20 rxns) | CB111-02 (60 rxns) |
| pEASY ®-Blunt Simple Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| SR Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
References
1 Wu X, Yu C, Mu W, et al. The structural mechanism for transcription activation by Caulobacter crescentus GcrA[J]. Nucleic Acids Research, 2023.(IF 19.16)
2 Li Y, Du Y, Huai J, et al. The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis[J]. The Plant Cell, 2022.(IF 12)
3 Wang X, Liu C, Zhang S, et al. N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles[J]. Developmental cell, 2021.(IF 10.09)
4 Wang H, Zhang L, Shang Y, et al. Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene[J]. Virus evolution, 2020.(IF 5.50)
5 Li L, Fang C, Zhuang N, et al. Structural basis for transcription initiation by bacterial ECF σ factors[J]. Nature communications, 2019.(IF 11.90)
6 Zhang W, Wan H, Feng G, et al. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys[J]. Nature, 2018.(IF 41.57)
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