pEASY®-Blunt3 Cloning Kit transgen CB301-01
$50.00
In stock
SKU
CB301-01
Introduction and Features
pEASY®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion. •5 minutes fast ligation of Pfu-amplified PCR products. •Ampicillin resistance gene for selection. •Easy blue/white selection. •T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. •T7 promoter and SP6 promoter for in vitro transcription. •Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
| Storage | Competent Cell at -70℃ for six months; others at -20℃ for 9 months |
| Shipping | Dry ice (-70℃) |
Product Composition
| Component | CB301-01 (20 rxns) | CB301-02 (60 rxns) |
| pEASY ®-Blunt3 Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
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