Introduction and Features
FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-15 minutes at 37°C, and heat-inactivated at 65°C for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.
Enzyme Properties
Fast digestion in 5-15 minutes with high fidelity
Application
Genomic DNA, plasmid DNA, PCR product
| Storage | at -20℃ for two years |
Product Composition
References
1.Lu C , Ma J , Cai D . Increased HAGLR expression promotes non–small cell lung cancer proliferation and invasion via enhanced de novo lipogenesis[J]. Tumor Biology, 2017, 39(4):101042831769757.
2.Jian‑Zhi Gao, Li J , Jing‑Li Du, et al. Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence[J]. Oncology letters, 2016, 11(3):1791-1798.
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