Introduction and Features
FlyCut® HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. The molecular weight is 35.8 kDa, with the recognition site at A^AGCTT. The reaction is conducted at 37oC, and heat inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
| Applications | Genomic DNA, plasmid DNA, PCR product |
| Storage | at -20oC for two years |
| Shipping | Dry ice (-70℃) |
Product Composition
| Component | JH101-01 | JH101-02 |
| FlyCut® HindIII | 5,000 units | 2×5,000 units |
| 10×FlyCut® Buffer | 2×1 ml | 3×1 ml |
| 10×DNA Loading Buffer | 1 ml | 1 ml |