Introduction and Features
FlyCut® NheI is expressed and purified from E.coli that carries the recombinant NheI gene. The molecular weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction is conducted at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Enzyme Properties
- Fast digestion in 5 minutes
- Universal buffer
- Star activity-free
Application
Genomic DNA, plasmid DNA, PCR product
| Storage | at -18℃ or below for two years |
| Shipping | Dry ice (-70℃) |
Product Composition
Reviews
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