Introduction and Features
FlyCut® SacI is expressed and purified from E.coli that carries the recombinant SacI gene. The molecular weight is 40.8 kDa, with the recognition site at GAGCT^C. The reaction is conducted at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Enzyme Properties
- Fast digestion in 5 minutes
- Universal buffer
- Star activity-free
Application
Genomic DNA, plasmid DNA, PCR product
| Storage | at -20℃ for two years |
Product Composition
Reviews
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