Introduction and Features
FlyCut® SacII is expressed and purified from E.coli that carries the recombinant SacII gene. The molecular weight is 32.1 kDa, with the recognition site at CCGC^GG. The reaction is conducted at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
| Storage | at -20oC for two years |
| Shipping | Dry ice (-70℃) |
Product Composition
| Component | JS201-01 | JS201-02 |
| FlyCut® SacII | 1,000 units | 2×1,000 units |
| 10×FlyCut® Buffer | 250 μl | 500 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |
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