Introduction and Features
FlyCut® SpeI is expressed and purified from E.coli that carries the recombinant SpeI gene. The molecular weight is 21.7 kDa, with the recognition site at A^CTAGT. The reaction is conducted for 5-15 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Application
Genomic DNA, plasmid DNA, PCR product
| Applications | Genomic DNA, plasmid DNA, PCR product |
| Storage | at -20℃ for two years |
Product Composition
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