Introduction and Features
FlyCut® Sphl is expressed and purified from E.coli that carries the recombinant Sphl gene. The molecular weight is 26.8 kDa, with the recognition site at GCATG^C. The reaction is conducted for 5-15 minutes at 37°C, and denatured at 65°C for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Enzyme Properties
Fast digestion in 5-15 minutes with high fidelity
Application
Genomic DNA, plasmid DNA, PCR product
| Applications | Genomic DNA, plasmid DNA, PCR product |
| Storage | at -20℃ for two years |
Product Composition
Reviews
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