Introduction and Features
FlyCut® Xbal is expressed and purified from E.coli that carries the recombinant Xbal gene. The molecular weight is 24.7 kDa, with the recognition site at T^CTAGA. The reaction is conducted at 37°C, and denatured at 65°C for 20 minutes. This enzyme is not sensitive to dcm or mammalian CpG methylation, but sensitive to dam methylation.
Enzyme Properties
- Fast digestion in 5 minutes
- Universal buffer
- Star activity-free
Application
Genomic DNA, plasmid DNA, PCR product
| Storage | at -20℃ for two years |
Product Composition
References
1 Wang Z P, Zhang Z B, Zheng D Y, et al. Efficient and genotype independent maize transformation using pollen transfected by DNA‐coated magnetic nanoparticles[J]. Journal of Integrative Plant Biology, 2022.(IF 7.00)
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