T7 Endonuclease I transgen LE101-01

$50.00
In stock
SKU
LE101-01
Catalog No.SizePrice (USD)
LE101-01250 units
LE101-025×250 units

Introduction and Features

This product is purified from E. coli expressing the recombinant T7 Endonuclease I (T7EI) gene. It recognizes and cleaves

non-perfectly matched DNA, cruciform DNA structures, Holliday junctions, heteroduplex DNA, and randomly cleaves

single‑stranded DNA. The cleavage site is at the first, second, or third phosphodiester bond that is 5´ to the mismatch.

Applications
  • Gene mutation and SNP detection for result from TALEN and CRISPR/CAS9
  • Recognition and cleavage for non-perfectly matched DNA and Holliday junctions
  • Randomly cleave single-stranded DNA
Storage

at -20oC for two years

Shipping

Dry ice (-70 ℃)

Product Composition

ComponentLE101-01LE101-02
T7 Endonuclease I250 units5×250 units
10×T7 Endonuclease I Buffer200 μl1 ml
T7 Endonuclease I Control Template (40 ng/μl)20 μl20 μl
10×DNA Loading Buffer1 ml1 ml
T7 Endonuclease I transgen LE101-01

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