Introduction and Features
Ribonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA. This product is purified from E. coli expressing the recombinant rnhA gene on a plasmid with 17 kDa molecular weight and it can be inactivated by heating at 65°C for 20 minutes.
Definition of Activity Unit One unit is defined as the amount of RNase H that solubilizes 1 nmol RNA in the 3 tagged poly(rA)·poly(dT) hybrid chain in 20 minutes at 37°C in 50 μl system.
| Applications | Removal of mRNA prior to synthesis of second strand cDNA. Identification of RNA-DNA Hybrid. |
| Storage | at -20°C for two years |
| Shipping | Dry ice (-70 ℃) |
Product Composition
| Component | LH101-01 | LH101-02 |
| RNase H | 100 units | 5×100 units |
| 10×RNase H Buffer | 1 ml | 5×1 ml |
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