T7 High Efficiency Transcription Kit transgen JT101-01
$50.00
In stock
SKU
JT101-01
Introduction and Features
This kit is designed to efficiently transcribe the DNA sequence at the downstream of the T7 promoter which is contained in supercoiled plasmid DNA or linear DNA as templates, with T7 RNA Polymerase in an optimized in vitro transcription reaction mix. It is suitable for the preparation of high-concentration RNA with a length of more than 6000 nt. Using 1 μg of DNA template, 150~280 μg of RNA can be generated in a 20 μl reaction mix (if milligram-level RNA products are required, the reaction mix can be scaled up in parallel). The prepared RNA can be used for in vitro translation, RNase protection assays, RNA shearing, and hybridization probe labeling.
| Storage | at -20°C for two years |
| Shipping | Dry ice (-70 ℃) |
Product Composition
| Component | JT101-01 (25 rxns) | JT101-02 (100 rxns) |
| T7 Transcription Enzyme Mix | 50 μl | 200 μl |
| 5×T7 Transcription Reaction Buffer | 100 μl | 400 μl |
| ATP (100 mM) | 50 μl | 200 μl |
| GTP (100 mM) | 50 μl | 200 μl |
| CTP (100 mM) | 50 μl | 200 μl |
| UTP (100 mM) | 50 μl | 200 μl |
| DNase I (1 unit/μl) | 50 μl | 200 μl |
| 500 mM EDTA (pH 8.0) | 25 μl | 100 μl |
| RNase-free Water | 1 ml | 5 ml |
| Transcription Control Template (0.5 μg/μl) | 10 μl | 40 μl |
References
1 Zhang Q, Wen F, Zhang S, et al. The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation[J]. Science advances, 2019.(IF 12.80)
2 Zhang Q, Chen Z, Wang F, et al. Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer[J]. Nucleic Acids Research, 2021.(IF 16.97)
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