2×TransTaq® High Fidelity (HiFi) PCR SuperMix II (-dye) transgen AS131-21

$50.00
In stock
SKU
AS131-21
Catalog No.SizePrice (USD)
AS131-211 ml
AS131-225×1 ml

Introduction and Features

This product is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase with hot start, dNTPs and optimized buffer. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O. Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T series vectors.

  • Reduced reaction setup time.
  • Minimized contamination caused by multi-step operation.
  • TransTaq® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
Applications
  • GC/AT-rich templates, complex templates and long PCR.
  • TransTaq® HiFi PCR SuperMix I is optimized for the amplification of genomic DNA (≤ 15 kb).
  • TransTaq® HiFi PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
Storage

-18°C or below for two years

Shipping

Dry ice (-70 ℃)

Product Composition

ComponentAS131-01/21AS131-02/22
2×TransTaq® HiFi PCR SuperMix (Mix I)/(Mix II)1 ml5×1 ml
Nuclease-free Water1 ml5 ml

References

1 Guo C J, Ma X K, Xing Y H, et al. Distinct processing of lncRNAs contributes to non-conserved functions in stem cells[J]. Cell, 2020.(IF 36.21)

2×TransTaq® High Fidelity (HiFi) PCR SuperMix II (-dye) transgen AS131-21

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