EasyTaq® DNA Polymerase (with 2.5 mM dNTPs) transgen AP111-11
$50.00
In stock
SKU
AP111-11
Introduction and Features
EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.
- Extension rate is about 1-2 kb/min. • Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors. • Amplification of genomic DNA fragment up to 4 kb.
| Applications |
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| Storage | at -20 ℃ for two years |
| Shipping | Dry ice (-70 ℃) |
Without dNTPs
| Component | AP111-01 | AP111-02 | AP111-03 | AP111-04 |
| EasyTaq ® DNA Polymerase | 500 U×1 | 500 U×6 | 2500 U×4 | 5000 U×10 |
| 10×EasyTaq ® Buffer | 1.2 ml×1 | 1.2 ml×6 | 1.2 ml×20 | 1.2 ml×100 |
| 6×DNA Loading Buffer | 1 ml×1 | 1 ml×2 | 1 ml×4 | 1 ml×20 |
With dNTPs
| Component | AP111-11 | AP111-12 | AP111-13 |
| EasyTaq ® DNA Polymerase | 500 U×1 | 500 U×6 | 2500 U×4 |
| 10×EasyTaq ® Buffer | 1.2 ml×1 | 1.2 ml×6 | 1.2 ml×20 |
| 2.5 mM dNTPs | 800 μl×1 | 800 μl ×6 | 800 μl×20 |
| 6×DNA Loading Buffer | 1 ml×1 | 1 ml×2 | 1 ml×4 |
References
Zhu C , Gao Y , Guo H , et al. Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution[J]. Cell Stem Cell, 2017, 20(5):720-731.
Shao N , Jiang S , Zhang M , et al. MACRO: A Combined Microchip-PCR and Microarray System for High-throughput Monitoring of Genetically Modified Organisms[J]. Analytical Chemistry, 2013, 86(2):1269-1276.
Li J , Fu J , Chen Y , et al. The U6, Biogenesis-Like 1, Plays an Important Role in Maize Kernel and Seedling Development by Affecting the 3′ End Processing of U6 snRNA[J]. Molecular Plant, 2017, 10(3):470-482.
Simond AM , Rao T , Zuo D , et al. ErbB2-positive mammary tumors can escape PI3K-p110α loss through downregulation of the Pten tumor suppressor[J]. Oncogene, 2017, 36(43):6059-6066.
Li Y , Guo SJ , Shao N , et al. A universal multiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray[J]. LAB ON A CHIP, 2011, 11(21):3609-8.
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