Introduction and Features
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent carry-over contamination.
| Applications |
|
| Storage | at -18℃ or below for two years |
| Shipping | Dry ice (-70 ℃) |
Product Composition
| Component | AQ322-01 | AQ322-02 |
| TransScript® II Multiplex Probe One-Step Enzyme Mix UDG | 80 μl | 320 μl |
| 2×PerfectStart® Multiplex Probe One-Step Reaction Mix | 1 ml | 4×1 ml |
| Passive Reference Dye (50×) | 40 μl | 160 μl |
| Nuclease-free Water | 1 ml | 4×1 ml |