TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG transgen AQ322-01

$50.00
In stock
SKU
AQ322-01
Catalog No.SizePrice (USD)
AQ322-01100 rxns×20 μl system
AQ322-02400 rxns×20 μl system

Introduction and Features

TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent carry-over contamination.

Applications
  • Singleplex to 4-plex RT-qPCR detection.
  • High-copy and low-copy gene detection.
  • RNA templates with high GC content or complex secondary structure.
  • Detection of RNA virus or trace amounts of RNA.
Storage

at -18℃ or below for two years

Shipping

Dry ice (-70 ℃)

Product Composition

ComponentAQ322-01AQ322-02
TransScript® II Multiplex Probe One-Step Enzyme Mix UDG80 μl320 μl
2×PerfectStart® Multiplex Probe One-Step Reaction Mix1 ml4×1 ml
Passive Reference Dye (50×)40 μl160 μl
Nuclease-free Water1 ml4×1 ml
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG transgen AQ322-01

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