PerfectStart® Plasmid DNA Quantification Kit transgen DH171-01
$50.00
In stock
SKU
DH171-01
Introduction and Features
This product is designed to target conserved sequences across various plasmids, enabling quantitative detection of residual plasmid DNA in a wide range of biological products by probe-based qPCR. It can be used together with magnetic bead-based host cell residual DNA extraction kit (Cat. No.: EH201). In addition, a dUTP/UDG system is introduced into this reaction mix, which can degrade U-containing ssDNA and dsDNA before reverse transcription, eliminating carry-over contamination caused by PCR products.
Features
- High specificity, high sensitivity, high amplification efficiency, and broad sample compatibility.
- Specially optimized qPCR reaction buffer provides faster extension speed, higher sensitivity and specificity.
- Incorporates UDG enzyme and dUTP to effectively prevent carry-over contamination from PCR products, ensuring accurate data.
| Storage | at -20°C for two years |
| Shipping | Dry ice (-70℃) |
Product Composition
| Component | DH171-01 | Main Ingredients |
| 2×Plasmid qPCR SuperMix | 2×750 μl | PCR enzyme, dNTPs, Mg2+, PCR buffer, etc. |
| 6×Plasmid Primer & Probe Mix | 500 μl | Plasmid DNA primer & probe |
| Linear Plasmid DNA Standard (2×10⁸ copies/μl) | 50 μl | |
| Circular Plasmid DNA Standard (2.5×10⁸ copies/μl) | 50 μl | |
| DNA Diluent | 3×1 ml | |
| Nuclease-free Water | 1 ml |
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