PerfectStart® HEK293 DNA Quantification Kit transgen DH152-01
$50.00
In stock
SKU
DH152-01
Introduction and Features
This product is designed for the quantitative detection of HEK293 cell DNA in various biological products by probe-based qPCR. It can be used together with magnetic bead-based host cell residual DNA extraction kit (Cat. No.: EH201). The 2×HEK293 qPCR SuperMix included in this product contains PerfectStart Taq hot start enzyme (which utilizes three monoclonal antibodies to efficiently bind with Taq DNA polymerase, effectively blocking the enzyme's activity and preventing non-specific amplification at low temperature), a specially optimized qPCR reaction buffer for HEK293 cell DNA detection, dNTPs, PCR enhancer, and stabilizer. In addition, a dUTP/UDG system is introduced into this reaction mix, which can degrade U-containing ssDNA and dsDNA before reverse transcription, eliminating carry-over contamination caused by PCR products.
Features
- Three-antibody blocking technology ensures high specificity, high sensitivity, high amplification efficiency, and broad sample compatibility.
- Specially optimized qPCR reaction buffer provides faster extension speed, higher sensitivity and specificity.
- Incorporates UDG enzyme and dUTP to effectively prevent carry-over contamination from PCR products, ensuring accurate data.
| Storage | at -20°C for two years |
| Shipping | Dry ice (-70℃) |
Product Composition
| Component | JH152-01 | Main Ingredients |
| 2×HEK293 qPCR SuperMix | 2× 750 μl | PCR enzyme, dNTPs, Mg2+, PCR buffer, etc. |
| 6× HEK293 Primer & Probe Mix | 500 μl | HEK293 cell DNA primer & probe |
| HEK293 DNA Standard (30 ng/μl) | 50 μl | HEK293 cell DNA |
| Standard Diluent | 3× 1 ml | |
| Nuclease-free Water | 1 ml |
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