TransScript® IV Visual One-Step gDNA Removal and cDNA Synthesis SuperMix transgen AW312-02
$50.00
In stock
SKU
AW312-02
Introduction and Features
TransScript® IV Reverse Transcriptase is a modified M-MLV reverse transcriptase. The product uses RNA as a template to synthesize first-strand cDNA while simultaneously removing residual genomic DNA from the RNA template within the same reaction system. After the reaction, heating at 85°C for 2 minutes can inactivate both the TransScript® IV RT/RI Enzyme Mix and the gDNA Remover simultaneously. The enzyme exhibits an optimal reaction temperature of 50°C and possesses ultra-high thermal stability. The product contains a blue tracking dye for visualizing pipetting and can be used in combination with the PerfectStart® IV Visual Green qPCR SuperMix (Cat. No. AQ641) to enable full visual tracking throughout both the reverse transcription and qPCR amplification workflow.
Features
- Wide range of applications: severely degraded RNA can be used as a template for reverse transcription.
- Strong inhibitor tolerance: excellent resistance to inhibitors remaining in RNA.
- Fast reaction speed: only 10 minutes for reverse transcription and gDNA removal.
- High processivity: synthesis length can be up to 20 kb.
- High thermal stability: reaction temperature 42°C–65°C.
- High sensitivity: high detection rate for trace RNA.
- After the reaction, TransScript® IV RT/RI Enzyme Mix and gDNA Remover are inactivated simultaneously. Compared with the traditional method of pretreating RNA with DNase I, it avoids the damage of RNA after treatment by DNase I.
- Blue tracking dye for pipetting visualization.
| Applications |
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| Storage | at -18℃ or below for two years |
| Shipping | Dry ice (-70℃) |
Product Composition
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